PeptideWADA ProhibitedCompare

TB-500

Also known as: Thymosin Beta-4, TB4, TB 500

1 · Natty510 · Enhanced

Lower numbers = closer to natural. Higher numbers = more enhanced.

Why this rating?

TB-500 (Thymosin Beta-4) is a naturally occurring peptide present in almost all human cells. It promotes systemic healing by upregulating actin and promoting cell migration, angiogenesis, and stem cell differentiation. Does not affect hormones, strength, or direct muscle building. Effects are purely therapeutic/regenerative. No PCT required. Rated 1 (basically natty) because faster healing only restores you toward baseline rather than pushing muscle, strength, or body composition beyond your natural ceiling.

Overview

Focuses on systemic healing, cell migration, and flexibility. A naturally occurring peptide present in almost all human cells that plays a key role in tissue repair and regeneration.

Important Warnings

•WADA-prohibited at all times (S2.4 growth factors and growth factor modulators) - unlike BPC-157, TB-500/Thymosin Beta-4 is explicitly banned and will fail a drug test
•"TB-500" is usually a short synthetic fragment, not full Thymosin Beta-4 - identity and purity vary by source
•Limited human clinical data - evidence is largely preclinical/animal

Purpose & Use Cases

Systemic Healing

Promotes healing throughout the body, not just at injection site.

Flexibility

Improves tissue flexibility and reduces adhesions.

Inflammation Reduction

Powerful anti-inflammatory effects.

Benefits

Systemic healing effects
Reduced inflammation
Improved flexibility
Enhanced wound healing
Promotes new blood vessel formation
Hair regrowth potential

Good to Know

The systemic half of the 'Wolverine' stack

BPC-157 + TB-500 is the classic 'Wolverine' healing stack. TB-500 is the systemic partner: it upregulates actin and drives cell migration/angiogenesis throughout the whole body, while BPC-157 is injected near a specific injury for local repair. Plasma half-life is actually short (hours), so the ~2x/week community dosing schedule is based on claims of prolonged tissue-level activity rather than lingering blood levels.

TB-500 is not actually full Thymosin Beta-4

'TB-500' sold as a research chemical is almost always a short synthetic fragment (commonly the actin-binding sequence around residues 17-23, LKKTETQ) of the 43-amino-acid natural Thymosin Beta-4, not the intact protein. The fragment carries most of the tissue-repair activity but lacks additional domains (including some immune functions) of full TB-4, and identity/purity vary by source.

Unlike BPC-157, TB-500 is banned by WADA

This is the key distinction for tested athletes: BPC-157 falls under WADA's S0 (non-approved substances), while Thymosin Beta-4 / TB-500 is explicitly named in the S2.4 Growth Factors and Growth Factor Modulators subsection and is prohibited at all times, causing a positive test. Do not assume the two healing peptides share the same anti-doping status.

Regenerative, not anabolic or hormonal

TB-500 does not affect the HPTA, does not aromatize, and builds no muscle or strength directly. It accelerates healing, reduces inflammation, and improves tissue flexibility, hence its natty rating of 2. Human clinical data is limited; most evidence is preclinical.

Dosage Guidelines

Experience Level	Dosage Range
Beginner	2 – 2.5 mg/week
Intermediate	2.5 – 5 mg/week
Advanced	5 – 6 mg/week

Frequency

2x/week during a 4-6 week loading phase, then often reduced to 1x/week for maintenance

Typical Cycle Length

6 – 12 weeks

Notes

Common community protocol: loading ~2-2.5mg twice weekly (4-6mg/week total) for 4-6 weeks, then maintenance of 2-2.5mg/week (often as a single weekly shot). This is a community-derived research-chemical protocol, not a clinically established regimen, published human trials of native thymosin β4 used much smaller mcg/kg IV or topical doses for wound healing, not this weekly-mg subcutaneous scale.

Half-Life

Plasma value from native Tβ4 animal/human PK data, no formal human PK studies exist for the synthetic TB-500 fragment sold commercially, and community claims of much longer tissue-level activity are unverified.

Side Effects

Fatigue

rare

Severity

1/5

Occasional tiredness.

Mitigation

Usually transient.

Head Pressure

rare

Severity

1/5

Temporary feeling of head pressure.

Mitigation

Usually resolves quickly.

General Mitigation Strategies

Very well-tolerated. Combine with BPC-157 for "Wolverine" synergy. Side effects are minimal and rare.

Support Supplements

Ancillary supplements commonly run alongside TB-500 to manage side effects, support the target tissue, or fill nutrient demands it creates.

Hydrolyzed collagen / gelatin + vitamin C

Provides the amino-acid substrate and vitamin C cofactor for collagen/extracellular-matrix synthesis, complementing TB-500's cell-migration and angiogenesis signaling during soft-tissue repair.

Dose: ~15g collagen/gelatin + ~50-200mg vitamin C
Timing: Around rehab/loading sessions
When: Most relevant when using TB-500 for tendon, ligament, or connective-tissue recovery.

Adequate protein intake

Repair and regeneration are protein-synthesis limited, a healing signal accomplishes little without sufficient dietary protein.

Dose: ~1.6-2.2 g/kg/day
Timing: Spread across the day

Post Cycle Therapy (PCT)

✓PCT Not Required

Does not affect hormones. No PCT required.

How It Works

TB-500 upregulates cell-building proteins like actin, promoting cell migration to injury sites. It reduces inflammation, promotes angiogenesis, and increases stem cell production and differentiation for tissue repair.